Helminthosporoside, a Host-specific Toxin from Helminthosporium sacchari

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Isolation and characterization of host-selective toxin from Helminthosporium sacchari.

Helminthosporium sacchari infects certain clones of sugar cane and produces a toxin with the same plant selectivity as the fungus itself. The toxin was purified by use of activated charcoal plus thin layer, gel, and ion exchange chromatography. Gas chromatography (GC) of a trimethylsilyl derivative of toxin gave a single peak. Toxin was characterized by GC, mass spectroscopy (MS), and NMR spect...

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Biological Activity of the Isomeric Forms of Helminthosporium sacchari Toxin and of Homologs Produced in Culture.

The effect of Helminthosporium sacchari (HS) toxin isomers and related, pathogen-produced compounds on dark CO(2) fixation in HS-susceptible sugar cane leaf slices was investigated. HS toxin consists of a mixture of three isomeric bis-5-O-(beta-galactofuranosyl)-beta-galactofuranosides (A, B, and C) differing in the position of one double bond in the sesquiterpene aglycone. Maximum inhibition o...

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Serinol as an activator of toxin production in attenuated cultures of Helminthosporium sacchari.

Successive transfer in synthetic medium of spores and mycelial fragments from original toxin-producing cultures of Helminthosporium sacchari results in attenuated cultures which do not produce the host-specific toxin helminthosporoside. When attenuated cultures are grown on material obtained from the water wash of sugarcane leaves susceptible to this fungus, the production of heminthosporoside ...

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Amino Acid Composition of the Host-specific Toxin of Helminthosporium carbonum.

The host-specific toxin of Helminthosporium carbonum (C(32)H(50)N(6)O(10)) was hydrolyzed by 6 n HCl to yield a number of alpha-amino acids. The common amino acids, proline and alanine, occurred in a ratio of 1:2. Two other unstable alpha-amino acids that produced lower color values with ninhydrin were also produced. One of these was tentatively identified as 2-amino-2,3-dehydro-3-methylpentano...

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Biochemical basis of the resistance of sugarcane to eyespot disease.

Helminthosporoside is the host-specific toxin produced by Helminthosporium sacchari, the organism causing eyespot disease on sugarcane. Clones of sugarcane susceptible to the toxin posses a membrane protein that binds the toxin. Membranes of resistant clones do not bind the toxin. In this study, a binding protein from a susceptible clone was compared with its counterpart from a resistant clone....

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1971

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)62091-5